Microbial enhanced oil recovery (MEOR) by Pseudomonas sp. under laboratory conditions
نویسندگان
چکیده
The purpose of this work was to propose sustainable solutions for advanced oil recovery by evaluating the ability bacterium Pseudomonas sp. in biotransformation alkanes, addition determining strain growth patterns under extreme conditions. For this, initially carried out laboratory conditions, which crude fractionated obtain saturated fraction used experiment. bacterial tolerance salinity and temperature also tested determine experimental conditions set up experiment regard these parameters. Additionally, an performed produce a biosurfactant through biostimulation. consisted triplicate with treatment control. treatments, Erlenmeyers flasks received 100 mL broth containing biosurfactant, 10 g (10%) NaCl, 3% 1% fraction. Erlenmeyer were incubated at 40 °C 180 rpm 18 days periodic analysis. results showed bacteria exhibited better °C, there no significant change different salinities, nonlimiting parameter. final experiment, analysed Optical Density (OD). low variation, lowest point T18 absorbance 0.115 highest T6 0.149. In qPCR analysis population, pattern found similar optical density results, variation; number copies 16S rRNA gene (6.66x 103) T0 T12 (7.86x 103). analysis, time 6 observed have rate, 54% (C30), followed 52% (C31) 51% (C29).
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ژورنال
عنوان ژورنال: Revista Eletrônica em Gestão, Educação e Tecnologia Ambiental
سال: 2023
ISSN: ['2236-1170']
DOI: https://doi.org/10.5902/2236117071814